In vitro fertilisation (IVF)

In vitro fertilisation (IVF) is based on the extraction of the woman’s eggs by means of ovarian puncture and then fertilising them in the laboratory with sperm from her partner (or a donor). The embryos generated will be cultured in the laboratory and their development will be monitored until they are ready to be transferred to the maternal uterus in order to achieve pregnancy. If enough viable or good-quality embryos are obtained, the surplus will be cryopreserved for future treatments.

There are two types of IVF:

Conventional IVF:
In the same culture dish, eggs and sperm are placed together, distributed in microdroplets of culture medium. The goal is for the sperm to be able to reach the eggs on their own and achieve fertilisation.

Intracytoplasmic sperm injection (ICSI):
In this case, the sperm is introduced directly into the egg by microinjection using a micromanipulation system.

This technique is more invasive and complex than artificial insemination, but its chances of success are higher, and it is the technique of choice in most cases of infertility.

Ovarian stimulation

The first step in this technique is to perform controlled ovarian stimulation to promote the production of mature eggs in order to obtain a greater number of them. To achieve this, hormonal treatment is necessary, which usually lasts around 10 days, culminating in the induction of ovulation by administering a trigger drug.

Ovarian puncture (egg retrieval)

Ovarian puncture or egg retrieval is performed 36 hours after ovulation induction. It is a surgical intervention performed under anaesthesia in which the gynaecologist uses a fine needle connected to an aspirator to access the ovaries through the vaginal cavity, guided by ultrasound, and aspirate the follicular fluid. The follicular fluid will be processed in the laboratory to wash and isolate the eggs. The semen sample is obtained by masturbation and is capacitated to perform conventional IVF or ICSI with the eggs obtained.

Incubation of embryos

The embryos obtained as a result of fertilisation are cultivated in the laboratory, inside an incubator that maintains optimal conditions for their correct development. Embryologists in the laboratory monitor this development for several days and select embryos that are of good quality to carry out the transfer or to be cryopreserved.

Embryo transfer to the uterus

To prepare for embryo transfer to the uterus, the patient receives an endometrial preparation hormonal treatment, so that the endometrium achieves the optimal appearance and thickness for embryo implantation. Using a transfer cannula, the gynaecologist deposits the embryo or embryos in the patient’s uterus. This last step is painless and does not require sedation.

Suitable for:

Moderate or severe cases of male infertility

Tubal factor (obstruction of the fallopian tubes)

Endometriosis

Low ovarian reserve

Advanced maternal age

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