Endometrial regeneration using Plasma Rich in Growth Factors

The objective of this treatment is the regeneration and repair of endometrial tissue using the patient’s own plasma. In this way, the aim is to generate an optimal uterine environment that favors embryo implantation.

The endometrium is the inner layer of the uterus, where the implantation of the embryo takes place to initiate pregnancy. In certain situations this tissue presents insufficient thickness and reduced receptivity. This regenerative treatment is applied in order to improve the state of the endometrium and, therefore, the embryo implantation environment.

To do this, we start with a blood sample from the patient herself. From this sample we obtain a Plasma Rich in Growth Factors, involved in the endometrial regeneration process.

Indications:

Those patients with endometrium refractory to usual treatment or repeated implantation failures are candidates.

Asherman's Syndrome

This disease is characterized by the appearance of adhesions or scars at the uterine level. The application of PRP can improve this situation thanks to the growth factors produced by platelets, which have a repairing and regenerating effect on the endometrium.

Refractory endometrium

There are situations in which the endometrium does not respond correctly to hormonal treatments, so that its thickness is insufficient to guarantee embryo implantation. The endometrium is considered to be refractory when its thickness is below 6 mm. Plasma Rich in Growth Factors can help us to increase this endometrial thickness.

Adenomyosis

In this pathology, there is an abnormal growth of endometrial tissue within the muscular layer of the uterus (myometrium). These cases can be improved by the application of Plasma Rich in Growth Factors.

Implantation failure

Finally, Plasma Rich in Growth Factors therapy may be indicated in cases of repeated implantation failure. If after several good quality embryo transfers implantation has not been achieved, this may be an adjuvant technique to improve the chances of pregnancy.

Phases of treatment

Evaluation of the case

First of all, the physician specialist in assisted reproduction must judge whether the case is indicative of endometrial regeneration therapy, because it fits any of the indications mentioned in the previous section.

Blood sampling

The platelet-enriched serum is obtained from the patient’s own blood, being an autologous therapy. Therefore, it is necessary to draw a blood sample from the patient to be treated.

Sample processing

Once the blood sample has been obtained, it must be processed in the laboratory to obtain the Plasma Rich in Growth Factors.

  1. The first step consists of centrifuging the blood to separate the different cell populations present in the blood.
  2. Once separated, the platelets and their growth factors are mixed with blood plasma to generate the Plasma Rich in Growth Factors fraction, which will be used in the treatment.

Application of Plasma Rich in Growth Factors

Plasma Rich in Growth Factors is applied directly into the uterus through a thin catheter that enters through the vagina. It is a painless process that does not require sedation and can be performed in the office, similar to an artificial insemination.

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Ángeles Bretón

CLINICAL EMBRYOLOGIST

Dr. Carlos Javier Vega Reina

GYNAECOLOGIST

Dr. María Miró

IMMUNOLOGIST

Dr. María Eugenia Molina

HEMATOLOGIST

Elena Mantrana Bermejo

Gynecologist. Specialist in Reproductive Medicine

  • Degree in Medicine and Surgery from the University of Seville.
  • Specialty in Obstetrics and Gynecology at Hospital Universitario de Valme, Seville.
  • Master’s Degree in Human Reproduction from the Universidad Rey Juan Carlos.
  • Specialist Physician of the Andalusian Health Service in the South Health Management Area (AGSS) of Seville.
  • Coordinator of the Assisted Human Reproduction Unit of the Hospital Universitario de Valme, Seville.
  • Member of the Human Reproduction Advisory Committee of AGSS of Seville.
  • Member of the working group for the update of the Guide for Assisted Human Reproduction of the Ministry of Health of the Andalusian Regional Government.
  • Tutor of Specialist Doctors in Training of the Teaching Area of the University Hospital of Valme.
  • Clinical Tutor Medical Students University of Seville

Alberto Armijo

Gynecologist. Reproductive Medicine Specialist